Signalling

Part:BBa_K511303:Design

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-27)


Delta-mCherry-4xFF4 Juxtacrine Signaling Ligand MammoBlock


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 85
    Illegal PstI site found at 162
    Illegal PstI site found at 1005
    Illegal PstI site found at 1428
    Illegal PstI site found at 2105
    Illegal PstI site found at 2491
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 85
    Illegal PstI site found at 162
    Illegal PstI site found at 1005
    Illegal PstI site found at 1428
    Illegal PstI site found at 2105
    Illegal PstI site found at 2491
    Illegal NotI site found at 494
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 85
    Illegal PstI site found at 162
    Illegal PstI site found at 1005
    Illegal PstI site found at 1428
    Illegal PstI site found at 2105
    Illegal PstI site found at 2491
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 85
    Illegal PstI site found at 162
    Illegal PstI site found at 1005
    Illegal PstI site found at 1428
    Illegal PstI site found at 2105
    Illegal PstI site found at 2491
    Illegal AgeI site found at 521
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 33
    Illegal BsaI site found at 473
    Illegal BsaI.rc site found at 1738
    Illegal BsaI.rc site found at 1910
    Illegal SapI site found at 1226
    Illegal SapI.rc site found at 1998


Design Notes

Due to the high secondary structure of FF4 miRNA, this part was assembled with standard restriction cloning methods, specifically using enzymes AscI and BamHI.


Source

This part is composed of the Delta-3 ligand from Rattus norvegicus and the synthetic mCherry fluorescent protein engineered from the green fluorescent protein isolated from Aequorea victoria; the sequence for the four FF4 micro RNA (miRNA) tags was synthetically produced.


References